Chemical Sterilization

At present, many chemical agents that have the potential to kill or inhibit microbes. Research and discovery of new chemical compounds continues to grow. A good agent is a chemical that has the ability to rapidly kill microbes with lower doses without damaging the material or equipment is disinfected.
In principle, the mechanism of chemical agents are classified into:

Physically sterilization

This method is used to sterilize equipment / material that resists heat, pressure or radiation. Sterilization can be done by:a. Dry heatDry heat, usually used to sterilize laboratory equipment. For tools such as Petri dishes, bottles or materials such as starch, talc and the like. Generally use the oven. Is the effective temperature of 160oC for 2 hours., Researchers often use temperature of 175oC for 1.5 to 2 hours. For tools such as tweezers needle loop, the tip of the pipette, test tube lip / erlenmeyer / petri dish sterilization typically use methylated spirit lamp or Bunsen.

Mechanic Sterilization

There are two filters, namely bacteriological filter and air filter.


a) bacteriological filters are typically used to sterilize materials that are not resistant to heating, such as sugar solution, serum, antibiotics, antitoxin, trace elements, enzymes, vitamins, hormones and or media containing materials etc.. Filtration technique using a filtering principle, which was filtered bacteria alone.