Inoculating with osse

a) Fuel needle loop of the base in the loop continues until the (end) until the red fluorescent

b) Allow for a few seconds until the glow disappeared, then immediately take a test tube containing the bacterial culture, the third to open the lid with the middle finger, ring and little fingers while the index finger and thumb holding the needle loop.

c) Fuel lip tube by rotating the tube so that all parts of the lips of the tubes exposed to flame

d) Immediately insert the needle into the loop tube, then immediately remove it. Try when inserting the needle ose not to touch the tube wall and do near the Bunsen burner

Pipetting

a) Take a sterile pipette, open the wrapper and put the rubber valve

b) Fuel burned edges on the Bunsen for a few seconds. Do not be too long because it can damage the tip of the pipette

c) Take the tube containing the bacterial culture,

open the lid with both ring and little fingers while the index finger, middle finger and thumb holding pipette.

d) Immediately insert the pipette into a test tube, rubber suction valve press the [S] (see figure below) and then immediately remove it. When inserting the pipe t try not to touch the walls of the tubes and Bunsen burners do in the near

Alcohol Flamming

Usually used to put paper discs or other instruments into a petri dish containing the media.

a) Take the forceps, dip the tip into the alcohol, and immediately above the tip Bunsen burn for a few seconds flat. Remember, do not skew

b) Take a paper disc with sterile forceps or other instruments were

c) Take a test tube containing the antimicrobial agent, the paper discs were dipped into the liquid in the test tube

d) Take a petri dish containing cultured bacteria that have been in the order, open the lid by tilting a few degrees up to only one side of the course is open. Remember do not be so wide open it or open the entire lid from the cup

e) Immediately insert the paper disc with sterile forceps or other instruments carefully in order not to damage the surface. Perform near Bunsen burner

f) Immediately cover the cup and lip burns again

g) Do it again in the same way if needed