Saturday, March 10, 2012

Alcohol Flamming

Usually used to put paper discs or other instruments into a petri dish containing the media.

a) Take the forceps, dip the tip into the alcohol, and immediately above the tip Bunsen burn for a few seconds flat. Remember, do not skew

b) Take a paper disc with sterile forceps or other instruments were

c) Take a test tube containing the antimicrobial agent, the paper discs were dipped into the liquid in the test tube

d) Take a petri dish containing cultured bacteria that have been in the order, open the lid by tilting a few degrees up to only one side of the course is open. Remember do not be so wide open it or open the entire lid from the cup

e) Immediately insert the paper disc with sterile forceps or other instruments carefully in order not to damage the surface. Perform near Bunsen burner

f) Immediately cover the cup and lip burns again

g) Do it again in the same way if needed

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