Inoculating with osse

a) Fuel needle loop of the base in the loop continues until the (end) until the red fluorescent

b) Allow for a few seconds until the glow disappeared, then immediately take a test tube containing the bacterial culture, the third to open the lid with the middle finger, ring and little fingers while the index finger and thumb holding the needle loop.

c) Fuel lip tube by rotating the tube so that all parts of the lips of the tubes exposed to flame

d) Immediately insert the needle into the loop tube, then immediately remove it. Try when inserting the needle ose not to touch the tube wall and do near the Bunsen burner


e) Burn back the lips and immediately close the tube. Remember, do not burn the needle loop back because it will kill the bacteria to be inoculated

f) Take another test tube to be inoculated, open the lid the same way by (b) and burn his lips in the same way by (c)

g) Immediately insert the needle into the tube was ose as means (d).

h) Fuel tube and close the lips as a way (c).

i) Fuel needle back loop as to (a).

j) Do it again in the same way if necessary dilution or dilution